sw620 human colorectal cancer cell line  (ATCC)

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Potential role of Adansonia digitata nanoparticles on colorectal cancer induced by colorectal cancer cells (SW620) in nude mice

doi: 10.1016/j.jgeb.2026.100659

Figure Lengend Snippet: Oxidative stress markers in nude mice colon treated with SW620, AD, ADNPs1, and ADNPs2. A significant change at p < 0.05 in MDA and a non-significant change at p > 0.05 in SOD and catalase activity. Values presented as mean ± S.E., a, b different superscripts within columns are significantly different ( P < 0.05).

Article Snippet: The SW620 human colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Potential role of Adansonia digitata nanoparticles on colorectal cancer induced by colorectal cancer cells (SW620) in nude mice

doi: 10.1016/j.jgeb.2026.100659

Figure Lengend Snippet: Anti-apoptotic/pro-apoptotic protein level markers in nude mice colon treated with SW620, AD, ADNPs1, and ADNPs2. A significant change at p < 0.05 compared to the SW620 group in cytochrome c and a non-significant change at p > 0.05 in caspase3, BAX, and BCL-2. Values presented as mean ± S.E., a, b different superscripts within columns are significantly different ( P < 0.05).

Article Snippet: The SW620 human colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques:

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Potential role of Adansonia digitata nanoparticles on colorectal cancer induced by colorectal cancer cells (SW620) in nude mice

doi: 10.1016/j.jgeb.2026.100659

Figure Lengend Snippet: TGF-β, TNF-α, INOS, and IL-1β mRNA expression in nude mice colon treated with SW620, AD, ADNPs1, and ADNPs2. A significant change at p < 0.05 compared to the SW620 group in TGF-β, and a non-significant change at p > 0.05 in TNF-α, IL-1β, and INOS. Values presented as mean ± S.E., a, b, c different superscripts within columns are significantly different ( P < 0.05).

Article Snippet: The SW620 human colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: CS-6-induced p62 accumulation exacerbates DNA damage in colorectal cancer

doi: 10.3389/fphar.2025.1568339

Figure Lengend Snippet: CS-6-mediated colorectal cancer suppression in vivo is associated with DNA damage and autophagy. (a) Tumor weight of SW620 cells in vivo. (b) Tumor volume of SW620 cells in vivo. (c) Body weight change of BALB/C nude mice. (d) Histological changes of liver and kidney in BALB/C nude mice treated with CS-6. (e) Expression of γH2AX and p62 was determined by immunohistochemistry (IHC). Data are presented as the mean ± SD from three independent experiments. (f) Expression of p-ATM, ATM, γH2AX, LC3 I/II and p62 in xenograft tumor at protein levels was detected by western blotting. *P < 0.05;; **P < 0.01; ***P < 0.001 compared with model group.

Article Snippet: The human colorectal cancer (CRC) cell lines, SW620 (ECACC identifier: 87051203) and DLD1 (ECACC identifier: 90102540) were obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: In Vivo, Expressing, Immunohistochemistry, Western Blot

Journal: Frontiers in Pharmacology

Article Title: CS-6-induced p62 accumulation exacerbates DNA damage in colorectal cancer

doi: 10.3389/fphar.2025.1568339

Figure Lengend Snippet: Schematic representation of the anti-tumor effect of CS-6 in colorectal cancer.

Article Snippet: The human colorectal cancer (CRC) cell lines, SW620 (ECACC identifier: 87051203) and DLD1 (ECACC identifier: 90102540) were obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques:

Journal: Advanced Science

Article Title: GRP78 Nanobody‐Directed Immunotoxin Activates Innate Immunity Through STING Pathway to Synergize Tumor Immunotherapy

doi: 10.1002/advs.202408086

Figure Lengend Snippet: GRP78 is overexpressed on the cell surface across various solid malignancies. a) Representative immunohistochemistry (IHC) staining for GRP78 illustrating elevated surface GRP78 expression in pan‐cancer tissues compared to low surface GRP78 expression in adjacent normal tissues. Scale bars, 300 µm, 40 µm, 10 µm (low to high magnification). The red arrows indicated the csGRP78. b) Representative immunofluorescence staining of csGRP78 in melanoma, breast cancer, and colorectal cancer cell lines. Scale bars, 50 µm.

Article Snippet: Human colorectal cancer cell lines (HT29, SW620), murine colorectal cancer cell lines (CT26, MC38), murine melanoma cell lines (B16, B16F1, B16F10), murine breast cancer cell line (4T1), murine dendritic cell line (DC2.4), and human embryonic kidney cell (HEK293) were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China) or ATCC (USA).

Techniques: Immunohistochemistry, Expressing, Immunofluorescence, Staining

Journal: Advanced Science

Article Title: GRP78 Nanobody‐Directed Immunotoxin Activates Innate Immunity Through STING Pathway to Synergize Tumor Immunotherapy

doi: 10.1002/advs.202408086

Figure Lengend Snippet: Anti‐GRP78 PE38 immunotoxin exerts potent antitumor effects against colorectal cancer and primary melanoma. a) Schematic of HT29 colorectal cancer model treated with PBS, Nb C5 (0.08 mg kg −1 ), or C5‐PE38 (0.3 mg kg −1 ) as indicated. BALB/c nude mice were injected subcutaneously with 5 × 10 6 HT29 tumor cells. b–d) Tumor growth and Kaplan‐Meier survival analysis of HT29 tumor‐bearing mice (mean ± SEM, n = 6–7 mice per group). Mice were euthanatized as an endpoint when the tumor volume reached 1500 mm 3 for survival analysis. e) Schematic of the B16F10 melanoma model treated with PBS, Nb C5, or C5‐PE38 as indicated. C57BL/6 mice were injected intradermally with 5 × 10 5 B16F10 tumor cells. f,g) Tumor growth of B16F10 tumor‐bearing mice following treatment (mean ± SEM, n = 4–5 mice per group). h) Tumor weight from B16F10 tumor‐bearing mice on day 9 post‐treatment (mean ± SEM, n = 4–5 mice per group). i) Representative images and quantification of Ki67 and TUNEL staining in tumor sections from the B16F10 tumor model following different treatments (mean ± SEM, n = 3 mice per group). H&E staining was performed for histological analysis. Scale bars, 100 µm (H&E), 50 µm (Ki67 and TUNEL). j) Representative immunofluorescence images and quantification of CALR staining in tumor sections from the B16F10 tumor model (mean ± SEM, n = 4 mice per group). Scale bars, 50 µm. Ki67‐positive, TUNEL‐positive, and CALR‐positive cell frequencies were quantified using ImageJ software. Statistical significance was assessed by two‐way ANOVA with Tukey's multiple‐comparisons (b, f), log‐rank Mantel‐Cox test (d), one‐way ANOVA with Tukey's multiple‐comparisons (h, i), and unpaired Student's t ‐test (j). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Human colorectal cancer cell lines (HT29, SW620), murine colorectal cancer cell lines (CT26, MC38), murine melanoma cell lines (B16, B16F1, B16F10), murine breast cancer cell line (4T1), murine dendritic cell line (DC2.4), and human embryonic kidney cell (HEK293) were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China) or ATCC (USA).

Techniques: Injection, TUNEL Assay, Staining, Immunofluorescence, Software

Journal: Biology

Article Title: Tumorspheres as In Vitro Model for Identifying Predictive Chemoresistance and Tumor Aggressiveness Biomarkers in Breast and Colorectal Cancer

doi: 10.3390/biology13090724

Figure Lengend Snippet: mRNA expression levels of inflammation-related markers in MCF7 ( A ) and SW620 ( B ) tumorspheres compared to a two-dimensional (2D) culture. Statistical significance was analyzed by Student’s t -test and set at * p ≤ 0.05.

Article Snippet: Human breast cancer cell line MCF7 and human colorectal cancer cell line SW620 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and grown in 100 mm culture dishes in DMEM supplemented with 10% FBS ( v / v ) and 1% penicillin and streptomycin ( v / v ).

Techniques: Expressing

Journal: Biology

Article Title: Tumorspheres as In Vitro Model for Identifying Predictive Chemoresistance and Tumor Aggressiveness Biomarkers in Breast and Colorectal Cancer

doi: 10.3390/biology13090724

Figure Lengend Snippet: Viability and tumorsphere formation efficiency of MCF7 ( A – D ) and SW620 ( E – H ) cell lines after Cisplatin/Oxaliplatin treatment, respectively. Statistical significance compared to 0 µM (Cisplatin or Oxaliplatin) was analyzed by Student’s t -test and set at * p ≤ 0.05.

Article Snippet: Human breast cancer cell line MCF7 and human colorectal cancer cell line SW620 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and grown in 100 mm culture dishes in DMEM supplemented with 10% FBS ( v / v ) and 1% penicillin and streptomycin ( v / v ).

Techniques: